Florida Nematode Sampling Instruction


Florida Nematode Sampling Instruction

While some nematode problems can be accurately diagnosed in the field, laboratory assay of soil and roots is usually necessary to confirm field observations. Please follow these instructions carefully for collecting and submitting a sample to us, so that it reaches us in good condition and you get accurate information from it. Remember, the accuracy of the results is directly related to the quality of the sample.

  1. The sample should be prepared from a mixture of 10 to 20 "cores" of soil. Cores are most easily taken with a soilsampling tube, auger, or trowel. A shovel may be used by cutting a 1-inch thick slice of soil through the soil profile, then collecting a 1 to 2-inch vertical band from the slice. It is often best to discard the top 1 inch from each core since nematode numbers may be very low there.

  2. Where to sample. Because nematodes feed on plant roots, always sample from among roots of the plants for which diagnosis is needed. Sample only when soil moisture is appropriate for working the field; avoid extremely dry or wet soil conditions. For the safety of lab personnel who handle the soil, Do Not Sample for at Least 4 Weeks after a Nematicide Has Been Applied to give the nematicide sufficient time to dissipate.

  3. Specific directions for collecting samples from different types of symptomatic plants are as follows:

    1. Annual Crops (most vegetables, annual ornamentals, and field crops) – take soil from root zones of 10-20 affected plants that are not yet dead. Include "feeder" or fine roots from several of them. Remove the surface inch of soil before taking each core 6-8 inches deep.

    2. Fruit and Nut Trees, Perennial Shrubs and Trees – if many plants are affected, include cores from several of them in the sample. If only one or a few are affected, take several cores from around each plant. Use a spade or shovel to dig within the "drip-line" (the area covered by the branches) to find fine feeder roots. Each "core" should consist of a few fine roots and soil from immediately around them. Discard roots and soil from the surface inch. On most trees and shrubs cores 8 inches deep should be sufficient. However, for burrowing nematode of citrus, collect roots and soil from below 12 inches deep.

    3. Turfgrasses - collect 10 to 20 cores from areas of declining but not yet dead turf. Collect cores 3-4 inches deep near the desired plant species, avoiding bare spots and weeds. The sample must consist of mostly soil with a few roots; discard foliage.

    Advisory or predictive samples are taken to predict the risk of nematode injury to a crop to be planted, or later on in the season for turfgrasses and other perennial crops. For annual crops nematodes are most numerous and easiest to detect near the end of a growing season. Therefore, sample results are generally more meaningful when the samples are collected immediately following the previous season's crop rather than immediately preceding the crop to be planted. Predictive samples should be representative of the entire sampling area. Collect cores in a regular pattern over the area. One sample should represent no more than 10 acres for relatively low-value crops such as corn and soybeans, and no more than 5 acres for higher valued crops such as vegetables and tobacco. Areas which have different soil types or which were planted to different crops (or varieties) during the past season should be sampled separately.

  4. Place all the cores from each sampled area into a plastic bag (You can use any zip top bag). The approximate soil volume for a properly collected sample should be 1 to 2 pints. Include as many fine roots as possible (up to ½ cup) mixed in with the soil sample. The soil should be handled carefully because rough handling will crush nematodes living among soil particles.

  5. Label the outside of the bag with your name and sample number or other identification so that the lab cannot confuse your sample with that of someone else. Label the bag by writing directly on it with a permanent black felt-tip marker or with a permanent pen or pencil on masking tape stuck to the bag. The paper tape enclosed in this kit to fasten the box together will not stick to the plastic; do not use it to label the bag. Do not place a paper label in the bag with the soil - it may decompose rapidly and be unreadable in a few days.

  6. Handling and Submission. Nematodes will die from overheating, freezing, or drying. Do not leave samples exposed to sunlight, or carry them in a hot car trunk or on the dashboard. Do not add water to the sample, even if it seems dry to you. Just package and send it so we get it in the same condition as when you collected it. If nematodes are killed in handling, they cannot be recovered in the laboratory, and you will receive false results.

  7. Complete the Nematode Assay Form with All Requested Information. Print or write clearly. Be sure that your sample identification on the bag and the information on the form are correctly matched. Complete information about cropping history and plans, symptoms, etc. will help us make a more accurate diagnosis and recommendation. Accurate identification of the plant species (and variety, if possible) for which a diagnosis is needed is absolutely necessary to make a recommendation.

  8. Payment. As of July 1, 2006, the fee is $20 for each sample from Florida and $25 for each sample from outside of Florida. Please do not send cash. Make check or money order payable to "University of Florida." RESULTS WILL NOT BE SENT TO YOU UNTIL THE FEE IS PAID. Please be sure that the name that appears in the sample identification information on the sample bag and on the Assay Form also appears on the check.

  9. Packing. Use the two pieces of gummed tape to assemble the enclosed pre-addressed box. Put the Assay Form, any correspondence about the problem, and payment in the envelope, and put it in the box with the plastic bag of soil. If you must send samples in zip-lock bags, please tape them shut after squeezing the air out of them, so soil will not spill or dry out. If submitting a number of samples, use the box as a guide to the sample size, even though it will be more convenient to ship samples in a larger carton. When shipping several samples together, separate them with newspaper to prevent the sample label from rubbing off in transit.

  10. Mail, ship, or deliver samples to the Nematode Assay Laboratory as quickly as possible. The mailing address is printed on the sample box and on the Nematode Assay Form. For those who prefer to deliver samples to the Laboratory, it is located on Building 970 Natural Area Drive. Gainesville, 32611, University of Florida campus. Telephone (352) 392-1994. Fax (352) 392-0190. Please deliver samples between 8:30 a.m. and 4:30 p.m.

  11. Services: We will determine the quantity of each kind of plant parasitic nematode recovered from the sample. These results and the appropriate recommendations will be written on the Nematode Assay Form. You and your County Extension agent will each be sent a copy of the Assay Form no later than 10 working days after receipt of the sample in the laboratory. In some instances, identifying nematodes to species is necessary to select the best management program. We will try to identify pests to species when necessary and possible. This may require 2-3 months additional time to culture the organism in question. When such a delay is required, the normal report will be returned within 10 days after sample receipt; a supplementary report will be provided when final results are available.

  12. For information concerning collecting and submission of samples or the status of samples already submitted contact:

For information concerning interpretation of results contact:
Tesfamariam Mekete Mengistu, PhD,
Research Nematologists
Nematode Assay Laboratory
University of Florida, 970 Natural Area Dr.
Gainesville, FL 32611-0820
Phone: (352) 392-1994
Fax: (352) 392-0190
E-mail: NEMALAB@ifas.ufl.edu, tmekete@ufl.edu

W. T. Crow, PhD, Landscape Nematologist
Department of Entomology and Nematology
University of Florida, PO Box 110620
Gainesville, FL 32611-0620
Phone: (352) 273-3941
Fax: (352) 392-0190
E-mail: WTCR@ufl.edu