This document was constructed and
is maintained by KHUONG
B. NGUYEN
Entomology
and Nematology Department
University of Florida
Use this table to identify species
of Steinernema and
choose appropriate enzymes
for PCR-RFLP
This document was constructed and
is maintained by KHUONG
B. NGUYEN
Entomology
and Nematology Department
University of Florida
Use this table to identify species
of Steinernema and
choose appropriate enzymes
for PCR-RFLP
How to identify species of Steinernema: Sequence the ITS region using primers 18S and 26S. Next, using GCG MAP and MAPSORT programs (GCG) to collect number of cuts, cut sites, and fragment lengths. Sequence length, number of cuts, and fragment lengths can be used to identify nematode species.
TABLE 1: Number of cuts (boldfaced numbers) for each restriction enzyme
and position of cuts (from pair 1) of ITS sequences of 10 species of Steinernema
cut by 22 enzymes.( TABLE )
Last Updated on 19 Sept 2016
By KHUONG
B. NGUYEN