All adult data are from the first generation

DIAGNOSTIC CHARACTERS

Species names are arranged in descending order of spicule length

Steinernema intermedium

Spicule length=91 um (84-100), IJ length=671 um (608-800), EP= 65 um (59-69), T=66 um (53-74), E%=96, mucron absent, dorsal depression of IJ tail. In male, mele D%=67 (58-76), SW=1.24 (1.03-1.39), GS=0.69 (0.62-0.77), spicule well curved with blunt tip, rostrum prominent, velum large.FIG.SEM,DNA

Steinernema arenarium (=anomalae)

Spicule length= 84 um (81-91), IJ length=1034 um (724-1408), EP=83 um (76-86), T=75 um (64-84), E%=119, hookless spicule with ball-like tip, D%=93 (male), SW=2.10, GS=0.65, in ventral view, cuneus of gubernaculum V-shaped, mucron absent. FIG.SEM,DNA .

Steinernema scapterisci

Spicule length=83 um (72-92), IJ length= 572 um (517-609), T=54 um (48-60), E%=73, in male, male D%=38 (32-44), SW= 2.52 (2.04-2.80), GS=0.78 (0.69-0.84), mucron present; female with large double-flapped epiptygma, and excretory duct with elliptically-shaped structure. FIG.SEM. DNA

Steinernema karii

Spicule length=83 um (73-91), IJ length=932 um (876-982), EP= 74 um (68-80), T=74 um (64-80), E%=96, mucron absent, male D%=66 (53-78), SW=1.50, GS=0.7, spicule well curved with blunt tip, spicule head twice as long as wide, velum thin; gubernaculum with posterior end bifurcate, cuneus present.

Steinernema diaprepesi   (new)

Spicule length=79 um (71-90), IJ length=1002 um (880-1133), EP=74 um (66-83), T=83 um (65-91), E%=89.6 (78-114), spicule head elongate, length/width=1.4-2.0, shaft very short, lamina thick, terminus blunt with longtudinal depression, velum present. D% of male=80 (68-86), SW=1.8 (1.5-2.0), GS=0.69 (0.59-0.79), epiptygma low, double flapped, male terminus without mucron, young female terminus usually with 5 papillae-like structures, dissapearing in fully mature females.   (SEM1)(SEM2) SEM3SEM4

Steinernema puertoricense

Spicule length=78 um (71-88), IJ length=1171 um (1057-1238), EP=95 um (90-102), T=94 um (98-107), E%=101, spicule well curved with tip without a hook, mucron absent, male D%=77, SW=1.52, GS=0.51, double flapped-epiptygma present in females; FIG.SEM.

Steinernema siamkayai

Spicule length=77.5 um (75-80), IJ length= 446 um (398-495) , EP= 35 um (29-38), T= 35.5 um (), E%=96 (85-112), spicule with prominent head, shaft indistinct, rostrum present; gubernaculum slender, tapering gradually posteriorly, male D%=42 (35-49), SW=1.7 (1.4-2.2), GS=0.7 (0.6-0.8) epiptygma present, mucron present.

Steinernema glaseri

Spicule length=77 um (64-90), IJ length=1130 um (864-1448) , EP=102 um (87-110), T=78 um (62-87), E%=131, spicule with prominent shaft and hooked tip, in ventral view, gubernaculum tapering gradually anteriorly, cuneus Y-shaped, male D%=70, SW=2.05 (1.64-2.43), GS=0.71 (0.64-0.85), epiptygma absent, mucron absent. FIG.SEM. DNA

Steinernema longicaudum

Spicule length=77 um (?), IJ length=1063 um (?), EP=81 um, T=95 um, E%=85, hookless spicule tip, male D%=62, SW=1.60, GS=0.62; spicule narrows suddenly to form tip with flattened terminus, epiptygma absent, mucron absent. FIG.SEM.

Steinernema caudatum

Spicule length=75 um, IJ length=1106 um (933-1296), EP=82 um (76-89), T=88 um (80-100), E%=94 (87-100), male D%=71, SW=2.22, GS=0.69. spicule tip truncate, mucron absent; female tail sometimes with 2 projections.

Steinernema thanhi (New)

Spicule length=72 um (67-78), IJ length=851 um (720-960), EP=75 um (68-84), T=63 um (52-72), E%=119 (101-138), male D%=73 (64-82), SW=1.8 (1.5-2.1), GS=0.7 (0.5-0.8), spicule head longer than wide, velum thin, mucron absent.  SEM1SEM2 SEM3

Steinernema oregonense

Spicule length=71 um (65-73), IJ length=980 um (820-1110), EP=66 um (60-72), T=70 um (64-78), E%=100 (90-110), male D%=73 (64-75), SW=1.51, GS=0.79, spicule moderately curved, spicule head enlarged, tapering anteriorly, mucron absent. DNA

Steinernema ceratophorum

Spicule length=71 um (54-90), IJ length=706 um (591-800), EP= 55 um (47-70), T=66 um (56-74), E%=84, male D%=51 (33-65), SW=1.4 (1.0-2.0), GS=0.6 (0.4-0.8), mucron absent in first and second generation, spicule curved with blunt tip, velum large extending nearly to spicule tip, gubernaculum boat-shaped with posterior end bifurcate, cuneus small.

Steinernema loci (New)

Spicule length=71 um (60-80), IJ length=986 um (896-1072), EP=80 um (71-86), T=75 um (66-83), E%=107 (94-120), male D%=73 (61-80), SW=1.9 (1.7-2.1), GS=0.7 (0.5-0.8), spicule head longer than wide, 1/3 of spicule length, tail without mucron. SEM1SEM2 SEM3

Steinernema affine

Spicule length=70 um (67-86), IJ length= 693 um (608-880), EP=62 um (51-69), T=66 um (64-74), E%=94, internal spine in IJ tail, male D%=61, SW=1.17, GS=0.66, mucron present, small. FIG.SEM.

Steinernema feltiae (=bibionis)

Spicule length=70 um (65-77) , IJ length= 849 um (736-950), EP=62 um (53-67), T=81 um (70-92), E%=78, male D%=60, mucron long, tail=81 m, SW=1.13 (0.9-1.30), GS%=59 (0.52-0.62), spicule slightly curved, spicule head twice as long as wide. FIG.SEM. DNA

Steinernema monticolum

Spicule length=70 um (61-80), IJ length=706 um (612-821), EP=58 um (54-62), T=77 um (71-95), E%=76 (63-86), male D%=55 (49-51), SW=1.40 (1.20-1.5), GS=0.60 (0.5-0.7), spicules wide, anterior end rounded, well curved, shaft short but distinct, velum large.

Steinernema ritteri

Spicule length=69 um (58-75), IJ length=510 um(470-590), EP=38 um (32-40), T=51um (44-56), E%=88, mucron absent, male D%=47, SW=1.56, GS=0.64, female with epiptygma.

Steinernema pakistanense (New)

Spicule length = 68 ± 3.6 um (62-73), labial region of IJ bearing two horn-like structures, IJ length= 683±21 um (649-716), EP=54±2.2 um (49-58), T=58±2.1 um (53-62), E%=91±5 (87-102), male D%=60 ± 3 (50-60), SW = 1.8 ± 0.1(1.0-2.2), GS = 0.6 ± 0.04 (0.5-0.6), spicule head elongate, broad not continuous with the blade, tip pointed, velum present, 10% of male with mucron.

Steinernema riobrave

Spicule length=67 um (63-75), IJ length= 622 um (561-701), EP=56 um (51-64), T=54 um (46-59), E%=105, spicule sickle-shaped, spicule head tapering anteriorly, in ventral view, width of neck of gubernaculum large, cuneus Y-shaped, mucron absent, male D%=71, SW=1.14, GS=0.76. FIG.SEM. DNA

Steinernema carpocapsae

Spicule length=66 um (58-77), IJ length=558 um (438-650), EP=38 um (30-60), T=53 um (46-61), E%=66, spicule head wider than long, ventrally projected, spicule tip pointed or bluntly pointed; in ventral view, cuneus arrowhead-shaped or Y-shaped, mucron present, male D%=41, SW about 1.72, GS=0.71. FIG.SEM. DNA

Steinernema bicornutum

Spicule length=65 um (53-70), IJ length=769 um (648-873), EP=61 um (53-65), T=72 um (63-78), E%=84 (80-100), male D%=52 (50-60), SW=2.22, GS=0.72, spicule curved, spicule head typified by ventral projection and a width greater than length, blade well curved anteriorly or at mid region, posterior part slightly curved ot straight, velum present; in ventral view, cuneus arrowhead-shaped, mucron absent. FIG.SEM. DNA

Steinernema abassi

Spicule length=65 um (57-74), IJ length=541 um (496-579), EP= 48 um (46-51), T=56 um (52-61), E%=86, male D%=60, SW=1.56 (1.07-1.87), GS=0.7 (0.58-0.85), mucron absent, spicule curved, velum large.Gubernaculum boat-shaped.

Steinernema kushidai

Spicule length= 63 um (48-72), IJ length=589 um (524-662), EP=46 um (42-50), T=50 um (44-59), E%=92; spicule head truncate or rounded anteriorly, almost as long as wide, blade with large anterior end, rostrum prominent, velum present; mucron absent, male D%=51; SW=1.50, GS=0.70. FIG.SEM. DNA

Steinernema sangi (New)

Spicule length=63 um (58-80), IJ length=753 um (704-784), EP=51 um (46-54), T=81 um (76-89), E%=62 (56-70), male D%=49 ((42-62), SW=1.5 (1.2-1.6). GS=0.6 (0.5-0.7), mucron small, spicule head short, velum large.  SEM1 SEM2 SEM3

Steinernema thermophilum (New)

spicule length=61 um (44-72), IJ length=555 um (510-620), EP=40 um (37-46), T=45 um (40-52), E%=90 (81-102), male D%=63 (50-87), SW=1.7 (1.2-2.8), GS=0.6(0.5-0.7), spicule head short, spicule tip pointed, velum present.

Steinernema cubanum

Spicule length=58 um (50-67), IJ length=1283 um (1149-1508), EP=106 um (101-114), T=67 um (61-77), E%=160, male D%=70, SW=1.40, GS=0.67, spicule head continuous with spicule blade dorsally, spicule tip hookless, in ventral view, cuneus of gubernaculum Y-shaped, velum narrow; mucron absent FIG.SEM.

Steinernema neocurtillae

Spicule length=58 um (52-64), IJ length=885 um (741-988), EP=18 um, E%=23, EP extremely short, T=80 um (64-97), male D%=12, SW=1.43 (1.18.1.64), GS=0.89 (0.82-0.93), spicule short, spicule tip with a prominent depression on ventral side; mucron present, short. FIG.SEM. DNA

Steinernema kraussei

Spicule length=49 um (42-53), IJ length= 951 um (797-1102), EP=63 um (56-66), T=79 um (63-86), E%=80, spicule well or moderately curved, mucron small, male D%=53, SW=1.10, GS=0.67. FIG.SEM.

Steinernema rarum

Spicule length=47 um (42-52), IJ length=511 um (443-573), EP=38 um (32-40), T=51 um (44-56), E%=72, morphometrics of this nematode are less than those of other steinernematids, mucron present, male D%=50, SW=0.94, GS=0.71. Spicules with rounded anterior end, shaft present, sometimes indistinct, velum wide. Gubernaculum thick, cuneus present.

Neosteinernema longicurvicauda

Spicule=61 um (52-67), IJ length=926 ) um (789-1084), EP=68 um (61-76), T=167 um (141-190), E%=41, male D%=44 (30-54), SW=1.03 (0.80-1.50), GS=0.97 (0.84-1.08), male terminus digitate, spicule foot-shaped, 27-29 genital papillae, phasmids large, female tail longer than ABW, IJ tail long, curved, as long as esophagus length. FIG.SEM.

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Updated October 2004

This document was constructed and is maintained by KHUONG B. NGUYEN

Entomology & Nematology Department

University of Florida